{"product_id":"purified-anti-human-mouse-beta-iii-tubulin-tu-20","title":"Purified Anti-Human\/Mouse Beta III Tubulin (TU-20)","description":"\u003cp\u003eThe antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: TU-20\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: Beta III Tubulin\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human|Mouse, Canine, Pig, Rat\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: Purified\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Mouse IgG1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: FC-IC, WB, IHC-P, ICC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light.\u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/project_elva_2k\/R8-91259+Rev.+A_TDS_Purified+Anti-HumanMouse+Beta+III+Tubulin+(TU-20).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eWestern blotting: Recommended dilution: 1-2 μg\/ml; positive control: porcine brain lysate; negative control: HPB-ALL human peripheral blood leukemia cell line; reducing conditions. \u003cbr\u003eImmunohistochemistry (paraffin sections): Recommended dilution: 10 μg\/ml, standard ABC technique (DAB+), pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or high temperature citrate buffer antigen retrieval; positive tissue: neuronal tissue. \u003cbr\u003eImmunocytochemistry: Positive material: Neuro2a mouse neuroblastoma cell line. \u003cbr\u003eFlow cytometry: Recommended dilution: 1-4 µg\/ml. Intracellular staining.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eREFERENCES\u003c\/b\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003eTheodorou E, et al. 2009. Genes Dev 23:575-88.\u003c\/li\u003e\n\u003cli\u003eDráberová E, et al. 2017. FASEB J 31:1828-1846.\u003c\/li\u003e\n\u003cli\u003eJirásek T, et al. 2009. Indian J Med Res 129:89-94.\u003c\/li\u003e\n\u003cli\u003eKukharskyy V, et al. 2004. Exp Cell Res 298:218-28.\u003c\/li\u003e\n\u003cli\u003eJirásek T, et al. 2007. Folia Histochem Cytobiol 45:41-5.\u003c\/li\u003e\n\u003cli\u003ePĕknicová J, et al. 2001. Biol Reprod 65:672-9.\u003c\/li\u003e\n\u003cli\u003eDráberová E, et al. 1998. Histochem Cell Biol 109:231-9.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e","brand":"Cytek Biosciences","offers":[{"title":"100 µg","offer_id":67679919865892,"sku":"R7-91259","price":226.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/R7-91259_FC_Histogram.png?v=1792021487","url":"https:\/\/cytekbio.com\/ja\/products\/purified-anti-human-mouse-beta-iii-tubulin-tu-20","provider":"Cytek Biosciences","version":"1.0","type":"link"}