{"product_id":"purified-anti-human-mouse-actin-muscle-hhf35","title":"Purified Anti-Human\/Mouse Actin (Muscle) (HHF35)","description":"\u003cp\u003eThe mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: HHF35\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: Actin (Muscle)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human|Mouse, Non-Human Primate, Canine, Chicken, Feline, Rabbit, Rat\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: Purified\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Mouse IgG1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: WB, IHC-P, ICC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light.\u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/project_elva_2k\/R8-91693+Rev.+A_TDS_Purified+Anti-HumanMouse+Actin+(Muscle)+(HHF35).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eWestern blotting: Recommended dilution: 1 μg\/ml; positive control: murine femoral muscle, murine heart, negative control: HUVEC line; reducing conditions. \u003cbr\u003eImmunohistochemistry (paraffin sections): Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eREFERENCES\u003c\/b\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003eMatsuyama A, et al. 2007. Hum Pathol 38:645-51.\u003c\/li\u003e\n\u003cli\u003eMatsuo S, et al. 2007. J Surg Oncol 96:633-6.\u003c\/li\u003e\n\u003cli\u003eTsukada T, et al. 1987. Am J Pathol 126:51-60.\u003c\/li\u003e\n\u003cli\u003eTsukada T, et al. 1987. Am J Pathol 127:389-402.\u003c\/li\u003e\n\u003cli\u003eSchmidt RA, et al. 1988. Am J Pathol 131:19-28.\u003c\/li\u003e\n\u003cli\u003eMiettinen M. 1988. Am J Pathol 130:205-15.\u003c\/li\u003e\n\u003cli\u003ede Sousa SO, et al. 2000. J Clin Pathol 53:153-6.\u003c\/li\u003e\n\u003cli\u003eHandharyani E, et al. 2001. J Vet Med Sci 63:547-52.\u003c\/li\u003e\n\u003cli\u003eIjzer J, et al. 2006. Comp Hepatol 5:7.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e","brand":"Cytek Biosciences","offers":[{"title":"100 µg","offer_id":67679916228644,"sku":"R7-91693","price":239.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/Symbol_Full_Color_-_JPG_RGB.jpg?v=1613537357","url":"https:\/\/cytekbio.com\/ja\/products\/purified-anti-human-mouse-actin-muscle-hhf35","provider":"Cytek Biosciences","version":"1.0","type":"link"}