{"product_id":"purified-anti-human-cd326-ep-cam-323-a3","title":"Purified Anti-Human CD326 (Ep-CAM) (323\/A3)","description":"\u003cp\u003eThe mouse monoclonal antibody 323\/A3 recognizes an extracellular epitope of CD326 \/ EpCAM, a marker of epithelial lineages, that is over-expressed in many carcinomas.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: 323\/A3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: CD326 (Ep-CAM)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: Purified\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Mouse IgG1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: FC, IP, WB, IHC-P, ICC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light.\u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/project_elva_2k\/R8-91671+Rev.+A_TDS_Purified+Anti-Human+CD326+(Ep-CAM)+(323A3).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eWestern blotting: Non-reducing conditions; recommended dilution: 1-2 μg\/ml; positive control: MCF-7 cells. \u003cbr\u003eImmunoprecipitation: Recommended dilution: 1-4 μg \/ ml per 100-500 μg of protein in 1 ml lysate. \u003cbr\u003eImmunohistochemistry (paraffin sections): Recommended dilution: 1-10 μg\/ml; pretreatment: tissue section digestion with pepsin (15 min at RT or 10 min at 37°C, 1 mg \/ ml Tris-HCl, pH 2.0); positive tissue: breast carcinoma. \u003cbr\u003eImmunocytochemistry: Recommended dilution: 1-10 μg\/ml.  \u003cbr\u003eFlow cytometry: Recommended dilution: 1-4 µg\/ml.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eREFERENCES\u003c\/b\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003eKirman I, et al. 2003. Dig Dis Sci 48:2306-9.\u003c\/li\u003e\n\u003cli\u003eWinter MJ, et al. 2003. Am J Pathol 163:2139-48.\u003c\/li\u003e\n\u003cli\u003eBalzar M, et al. 2001. Mol Cell Biol 21:2570-80.\u003c\/li\u003e\n\u003cli\u003eBalzar M, et al. 1998. Mol Cell Biol 18:4833-43.\u003c\/li\u003e\n\u003cli\u003eLitvinov SV, et al. 1994. Cell Adhes Commun 2:417-28.\u003c\/li\u003e\n\u003cli\u003eLitvinov SV, et al. 1994. J Cell Biol 125:437-46.\u003c\/li\u003e\n\u003cli\u003eEdwards DP, et al. 1986. Cancer Res 46:1306-17.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e","brand":"Cytek Biosciences","offers":[{"title":"100 µg","offer_id":67679890636836,"sku":"R7-91671","price":173.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/R7-91671_FC_Histogram.png?v=1792021181","url":"https:\/\/cytekbio.com\/ja\/products\/purified-anti-human-cd326-ep-cam-323-a3","provider":"Cytek Biosciences","version":"1.0","type":"link"}