{"product_id":"purified-anti-human-cd11b-mem-174","title":"Purified Anti-Human CD11b (MEM-174)","description":"\u003cp\u003eThe antibody MEM-174 recognizes an extracellular epitope of CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: MEM-174\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: CD11b\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: Purified\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Mouse IgG2a\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: FC, IP\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light.\u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/project_elva_2k\/R8-91165+Rev.+A_TDS_Purified+Anti-Human+CD11b+(MEM-174).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eFlow cytometry: Recommended dilution: 1 μg\/ml; positive control: peripheral blood lymphocytes. \u003cbr\u003e Immunoprecipitation: Positive control: granulocytes, CD11b\/CD18 transfectants. \u003cbr\u003eImmunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody\/1 ml of sorbent gel (e.g. agarose beads). It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eREFERENCES\u003c\/b\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003eHentzen ER, et al. 2000. Blood 95:911-20.\u003c\/li\u003e\n\u003cli\u003eLawrence PK, et al. 2007. Vet Immunol Immunopathol 119:287-98.\u003c\/li\u003e\n\u003cli\u003eAkramiene D, et al. 2007. Medicina (Kaunas) 43:597-606.\u003c\/li\u003e\n\u003cli\u003eDrbal K, et al. 2007. Int Immunol 19:675-84.\u003c\/li\u003e\n\u003cli\u003eHasan S, et al. 2015. FEBS Lett 589:374-9.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e","brand":"Cytek Biosciences","offers":[{"title":"100 µg","offer_id":67679884279844,"sku":"R7-91165","price":173.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/R7-91165_FC_Profile.png?v=1792021008","url":"https:\/\/cytekbio.com\/ja\/products\/purified-anti-human-cd11b-mem-174","provider":"Cytek Biosciences","version":"1.0","type":"link"}