{"product_id":"purified-anti-human-beta-2-microglobulin-b2m-01","title":"Purified Anti-Human Beta-2 Microglobulin (B2M-01)","description":"\u003cp\u003eThe mouse monoclonal antibody B2M-01 reacts with beta2-microglobulin (beta2M; extracellular antigen), associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: B2M-01\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: Beta-2 Microglobulin\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: Purified\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Mouse IgG2a\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: FC, IP, WB, IHC-P, ICC, ELISA, RIA\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light.\u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/project_elva_2k\/R8-91215+Rev.+A_TDS_Purified+Anti-Human+Beta-2+Microglobulin+(B2M-01).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eWestern blotting: Recommended dilution: 2-4 μg\/ml; positive control: U937 human histiocytic lymphoma cell line, RAJI human Burkitt lymphoma cell line, negative control: EL4 mouse lymphoblastic lymphoma cell line; both reducing and non-reducing conditions. \u003cbr\u003eImmunohistochemistry (paraffin sections): Recommended dilution: 10 μg\/ml; positive tissue: kidney glomeruli. \u003cbr\u003eFlow cytometry: Recommended dilution: 0.5-4 μg\/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes. \u003cbr\u003eELISA: Working dilution should be determinated by investigator. \u003cbr\u003eRIA: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10\u003csup\u003e-8\u003c\/sup\u003e mol\/l as determined by competitive RIA. \u003cbr\u003eImmunoprecipitation: The antibody B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cells.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eREFERENCES\u003c\/b\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003ePoláková K, et al. 2013. Leuk Res 37:139-45.\u003c\/li\u003e\n\u003cli\u003eKhurana M, et al. 2006. Pediatr Nephrol 21:1257-65.\u003c\/li\u003e\n\u003cli\u003eHilgert I, et al. 1984. Folia Biol (Praha) 30:369-76.\u003c\/li\u003e\n\u003cli\u003ePolakova K, et al. 1993. Mol Immunol 30:1223-30.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e","brand":"Cytek Biosciences","offers":[{"title":"100 µg","offer_id":67679879790628,"sku":"R7-91215","price":173.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/R7-91215_FC_Profile.png?v=1792020969","url":"https:\/\/cytekbio.com\/ja\/products\/purified-anti-human-beta-2-microglobulin-b2m-01","provider":"Cytek Biosciences","version":"1.0","type":"link"}