{"product_id":"phospho-zap70-syk-tyr319-tyr352-monoclonal-antibody-n3kobu5-percp-efluor-710-ebioscience","title":"Phospho-ZAP70\/Syk (Tyr319, Tyr352) Monoclonal Antibody (n3kobu5), PerCP-eFluor 710, eBioscience","description":"\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost: \u003c\/b\u003e\u003cspan\u003eMouse\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype: \u003c\/b\u003e\u003cspan\u003eIgG2b, kappa\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality: \u003c\/b\u003e\u003cspan\u003eMonoclonal\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone: \u003c\/b\u003e\u003cspan\u003en3kobu5\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat: \u003c\/b\u003e\u003cspan\u003ePerCP-eFluor 710\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity: \u003c\/b\u003e\u003cspan\u003eHu, Ms\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication: \u003c\/b\u003e\u003cspan\u003eFlow Cytometry\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution: \u003c\/b\u003e\u003cspan\u003e5 µL (0.03 µg)\/test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration: \u003c\/b\u003e\u003cspan\u003e5 μL\/Test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage: \u003c\/b\u003e\u003cspan\u003e4°C, store in dark, DO NOT FREEZE!\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation: \u003c\/b\u003e\u003cspan\u003ePBS with BSA and 0.09% sodium azide; pH 7.2\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification: \u003c\/b\u003e\u003cspan\u003eAffinity chromatography\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheet: \u003c\/b\u003e\u003ca href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/dataSheetPdf?producttype=antibody\u0026amp;productsubtype=antibody_primary\u0026amp;productId=46-9006-42\" target=\"_blank\"\u003eTDS\u003c\/a\u003e\u003c\/p\u003e \u003cbr\u003e\u003cp\u003e\u003cspan\u003e\u003cb\u003eSpecific Information\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003eDescription: This n3kobu5 monoclonal antibody recognizes human and mouse zeta chain-associated protein of 70 kD (also known as ZAP-70) and spleen tyrosine kinase (also known as SYK) when phosphorylated on Y319 and Y352, respectively. ZAP-70 and SYK are members of the SYK protein tyrosine kinase (PTK) family that are phosphorylated and activated by Src family PTKs. ZAP-70\/SYK Y319\/Y352 are located in the so-called interdomain of ZAP-70\/SYK (between the N-terminal dual SH2 domains and the C-terminal kinase domain).\u003c\/p\u003e\u003cp\u003ePhosphorylation of ZAP-70 Y319 by Lck is necessary for T cell receptor (TCR)-dependent association of ZAP-70 with Lck and phospholipase C gamma and subsequent activation of calcium-dependent and Ras signaling cascades. SYK Y352 phosphorylation by Fyn\/Lyn is critical for propagation of B cell receptor (BCR) signaling and for B cell development.\u003c\/p\u003e\u003cp\u003eSpecificity of this n3kobu5 clone was determined by ELISA, flow cytometry, and western blotting.\u003c\/p\u003e\u003cp\u003eApplications Reported: This n3kobu5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.\u003c\/p\u003e\u003cp\u003eApplications Tested: This n3kobu5 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells\/test.\u003c\/p\u003e\u003cp\u003eStaining Protocol: All protocols work well for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation\/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to \"Clone Performance Following Fixation\/Permeabilization\" located in the BestProtocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: \"Staining Intracellular Antigens for Flow Cytometry Protocol\" located in the \u003ca href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/references\/protocols\/cell-and-tissue-analysis\/protocols\/staining-intracellular-antigens-flow-cytometry.html?cid=bid_pca_imm_r01_co_cp1600_pgm0000_intprotocol_0wm_tfs_il_edu_an_s00_\"\u003eBestProtocols®\u003c\/a\u003e Section under the Resources tab online.\u003c\/p\u003e\u003cp\u003ePerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710\/50 bandpass filter, however, the 695\/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.\u003c\/p\u003e\u003cp\u003eLight sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.\u003c\/p\u003e\u003cp\u003eFixation: Samples can be stored in IC Fixation Buffer (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/00-8222-49?SID=srch-srp-00-8222-49\"\u003eProduct # 00-8222\u003c\/a\u003e) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix\/Lyse Solution (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/00-5333-54?SID=srch-hj-00-5333-54\"\u003eProduct # 00-5333\u003c\/a\u003e) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency\/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.\u003c\/p\u003e\u003cp\u003eExcitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.\u003c\/p\u003e\u003cp\u003eFiltration: 0.2 µm post-manufacturing filtered.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eFor Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.","brand":"Cytek Biosciences","offers":[{"title":"100 Tests","offer_id":43191007641636,"sku":"46-9006-42","price":504.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/46-9006-42_Phospho-ZAP70-Syk-Tyr319-Tyr352_Flow_1-20170119083539.jpg?v=1769045731","url":"https:\/\/cytekbio.com\/ja\/products\/phospho-zap70-syk-tyr319-tyr352-monoclonal-antibody-n3kobu5-percp-efluor-710-ebioscience","provider":"Cytek Biosciences","version":"1.0","type":"link"}