{"product_id":"pe-cyanine7-anti-human-mouse-helios-22f6","title":"PE-Cyanine7 Anti-Human\/Mouse Helios (22F6)","description":"\u003cp\u003eThe Armenian hamster monoclonal antibody 22F6 recognizes Helios, a transcription factor (intracellular antigen) expressed in some hematopoietic stem cells, and at high levels in thymic-derived regulatory T cells. The epitope is located between amino acids 51 and 107.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: 22F6\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: Helios\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human|Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: PE-Cyanine7\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Armenian Hamster IgG\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution\u003c\/b\u003e: 5 µL\/Test\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: FC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light.\u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/project_elva_2k\/R8-95139+Rev.+A_TDS_PE-Cyanine7+Anti-HumanMouse+Helios+(22F6).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eFlow cytometry:  The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eREFERENCES\u003c\/b\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003eSerre K, et al. 2011. PLoS One 6:e20731.\u003c\/li\u003e\n\u003cli\u003eThornton AM, et al. 2010. J Immunol 184:3433-41.\u003c\/li\u003e\n\u003cli\u003eMcIver Z, et al. 2013. Haematologica 98:346-52.\u003c\/li\u003e\n\u003cli\u003eAtarashi K, et al. 2013. Nature 500:232-6.\u003c\/li\u003e\n\u003cli\u003eBlankenhaus B, et al. 2014. PLoS Pathog 10:e1003913.\u003c\/li\u003e\n\u003cli\u003eDaley SR, et al. 2013. J Exp Med 210:269-85.\u003c\/li\u003e\n\u003cli\u003ePinheiro D, et al. 2011. Immunology 132:111-22.\u003c\/li\u003e\n\u003cli\u003eFourcade J, et al. 2018. JCI Insight 3.\u003c\/li\u003e\n\u003cli\u003eČerný V, et al. 2021. Biomedicines 9.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003ePE-Cyanine7 is a tandem dye made with PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.\u003c\/p\u003e","brand":"Cytek Biosciences","offers":[{"title":"100 Tests","offer_id":67679870386212,"sku":"R7-95139","price":332.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/Symbol_Full_Color_-_JPG_RGB.jpg?v=1613537357","url":"https:\/\/cytekbio.com\/ja\/products\/pe-cyanine7-anti-human-mouse-helios-22f6","provider":"Cytek Biosciences","version":"1.0","type":"link"}