{"product_id":"pe-cyanine7-anti-human-cd152-ctla-4-bni3","title":"PE-Cyanine7 Anti-Human CD152 (CTLA-4) (BNI3)","description":"\u003cp\u003eThe mouse monoclonal antibody clone BNI3 binds to human CD152, commonly known as CTLA-4, a 33-37 kDa protein expressed as a homodimer on the surface of activated T and B cells, and on thymocytes. CTLA-4 is structurally similar to the T cell co-stimulatory molecule CD28, yet functionally disparate. Both CTLA-4 and CD28 interact with the co-stimulatory molecules CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells, with CTLA-4 displaying a higher avidity than CD28. While CD28 typically delivers a potent co-stimulatory signal in support of T cell activation, CTLA-4 appears to act as a negative regulator of T cell activation and may contribute to the suppressor function of Treg cells. CTLA-4 proteins may be initially sequestered within Golgi vesicles, from which they can be transferred to and from the cell surface, a mechanism by which Treg cells can selectively impart suppressive functions.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: BNI3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: CD152 (CTLA-4)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human, Baboon, Cynomolgus Monkey, Rhesus Macaque\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: PE-Cyanine7\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Mouse IgG2a, k\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution\u003c\/b\u003e: 5 µL\/Test\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: FC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light.\u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/project_elva_2k\/R8-95151+Rev.+A_TDS_PE-Cyanine7+Anti-Human+CD152+(CTLA-4)+(BNI3).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eFlow cytometry:  The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eREFERENCES\u003c\/b\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003eCastan J, Tenner-Racz K, et al. 1997. Immunology. 90:265-271.\u003c\/li\u003e\n\u003cli\u003eZola H. 2007. Leukocyte and stromal cell molecules: the CD markers. Hoboken, N.J.: Wiley-Liss.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003ePE-Cyanine7 is a tandem dye made with PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.\u003c\/p\u003e","brand":"Cytek Biosciences","offers":[{"title":"100 Tests","offer_id":67679868485668,"sku":"R7-95151","price":332.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/R7-95151_FC_Histogram.png?v=1792020764","url":"https:\/\/cytekbio.com\/ja\/products\/pe-cyanine7-anti-human-cd152-ctla-4-bni3","provider":"Cytek Biosciences","version":"1.0","type":"link"}