{"product_id":"mouse-igg2a-kappa-isotype-control-ebm2a-brilliant-violet-711-ebioscience","title":"Mouse IgG2a kappa Isotype Control (eBM2a), Brilliant Violet 711, eBioscience","description":"\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost: \u003c\/b\u003e\u003cspan\u003eMouse\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype: \u003c\/b\u003e\u003cspan\u003eIgG2a, kappa\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality: \u003c\/b\u003e\u003cspan\u003eMonoclonal\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone: \u003c\/b\u003e\u003cspan\u003eeBM2a\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat: \u003c\/b\u003e\u003cspan\u003eBrilliant Violet 711\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity: \u003c\/b\u003e\u003cspan\u003eN\/A\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication: \u003c\/b\u003e\u003cspan\u003eFlow Cytometry\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution: \u003c\/b\u003e\u003cspan\u003eAssay-dependent\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration: \u003c\/b\u003e\u003cspan\u003e0.2 mg\/mL\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage: \u003c\/b\u003e\u003cspan\u003e4°C, store in dark, DO NOT FREEZE!\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation: \u003c\/b\u003e\u003cspan\u003ePBS with BSA and 0.09% sodium azide; pH 7.2\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification: \u003c\/b\u003e\u003cspan\u003eAffinity chromatography\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheet: \u003c\/b\u003e\u003ca href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/dataSheetPdf?producttype=antibody\u0026amp;productsubtype=antibody_primary\u0026amp;productId=407-4724-81\" target=\"_blank\"\u003eTDS\u003c\/a\u003e\u003c\/p\u003e \u003cbr\u003e\u003cp\u003e\u003cspan\u003e\u003cb\u003eSpecific Information\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003eDescription: This is a monoclonal mouse IgG2a kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.\u003c\/p\u003e\u003cp\u003eApplications Reported: This eBM2a antibody has been reported for use in flow cytometric analysis.\u003c\/p\u003e\u003cp\u003eApplications Tested: This eBM2a antibody has been tested by flow cytometric analysis of normal human peripheral blood and mouse splenocytes. Use at the same concentration as the experimental antibody.\u003c\/p\u003e\u003cp\u003eBrilliant Violet™ 711 (BV711) is a tandem dye that emits at 713 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.\u003c\/p\u003e\u003cp\u003eWhen using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/SB-4401-42\"\u003eProduct # SB-4401-42\u003c\/a\u003e) or Brilliant Stain Buffer™ (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/00-4409-75\"\u003eProduct # 00-4409-75\u003c\/a\u003e) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.\u003c\/p\u003e\u003cp\u003eLight sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.\u003c\/p\u003e\u003cp\u003eFixation: Samples can be stored in IC Fixation Buffer (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/00-8222-49\"\u003eProduct # 00-8222-49\u003c\/a\u003e) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix\/Lyse Solution (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/00-5333-54\"\u003eProduct # 00-5333-54\u003c\/a\u003e) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency\/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically.\u003c\/p\u003e\u003cp\u003eOur internal testing suggests that Brilliant Violet™ 711 (BV711) is not compatible with methanol-based fixation.\u003c\/p\u003e\u003cp\u003eExcitation: 407 nm; Emission: 713 nm; Laser: Violet Laser.\u003c\/p\u003e\u003cp\u003eBRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eFor Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.","brand":"Cytek Biosciences","offers":[{"title":"50 µg","offer_id":43190998761508,"sku":"407-4724-81","price":254.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/407-4724-mouseigg2akappa-flow-cytometry-06may2022aur3vm-ebm2a-bv711-abmaint_20220603193513.jpg?v=1769045519","url":"https:\/\/cytekbio.com\/ja\/products\/mouse-igg2a-kappa-isotype-control-ebm2a-brilliant-violet-711-ebioscience","provider":"Cytek Biosciences","version":"1.0","type":"link"}