{"product_id":"lyve1-monoclonal-antibody-aly7-pe-ebioscience","title":"LYVE1 Monoclonal Antibody (ALY7), PE, eBioscience","description":"\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost: \u003c\/b\u003e\u003cspan\u003eRat\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype: \u003c\/b\u003e\u003cspan\u003eIgG1, kappa\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality: \u003c\/b\u003e\u003cspan\u003eMonoclonal\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone: \u003c\/b\u003e\u003cspan\u003eALY7\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat: \u003c\/b\u003e\u003cspan\u003ePE\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity: \u003c\/b\u003e\u003cspan\u003eMs\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication: \u003c\/b\u003e\u003cspan\u003eFlow Cytometry\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution: \u003c\/b\u003e\u003cspan\u003e0.125 µg\/test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration: \u003c\/b\u003e\u003cspan\u003e0.2 mg\/mL\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage: \u003c\/b\u003e\u003cspan\u003e4°C, store in dark, DO NOT FREEZE!\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation: \u003c\/b\u003e\u003cspan\u003ePBS with 0.09% sodium azide; pH 7.2\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification: \u003c\/b\u003e\u003cspan\u003eAffinity chromatography\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheet: \u003c\/b\u003e\u003ca href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/dataSheetPdf?producttype=antibody\u0026amp;productsubtype=antibody_primary\u0026amp;productId=12-0443-82\" target=\"_blank\"\u003eTDS\u003c\/a\u003e\u003c\/p\u003e \u003cbr\u003e\u003cp\u003e\u003cspan\u003e\u003cb\u003eSpecific Information\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003eDescription: The monoclonal antibody ALY7 recognizes mouse LYVE-1, a transmembrane glycoprotein with similarity to CD44. The extracellular domain contains a conserved hyaluronan binding domain also found in CD44. Expression is found on lymphatic and liver endothelial cells and some populations of macrophages. The lymphatic system is responsible for transporting proteins and cells (especially dendritic cells) to tissues throughout the body, thereby acting as immune surveyors. LYVE-1 is one characteristic protein, along with podoplanin, PROX-1, Tie-2 and VEGFR-3, that is expressed on lymphatic endothelial cells (LECS). The ligand for LYVE-1 is hyaluronan, a large mucopolysaccharide. Although LYVE-1 can bind hyaluronan in vitro, the site for ligand binding in vivo is masked by sialyated O-linked glycan chains. It is postulated that binding to ligand requires modification\/unmasking to expose the binding site. The development and remodeling of the endothelium after injury is an area of extensive study. When transplanted, hematopoietic stem cells (HSCs) can give rise to LECs that integrate into the endothelium in normal and metastatic tissue.\u003c\/p\u003e\u003cp\u003eApplications Reported: This ALY7 antibody has been reported for use in flow cytometric analysis.\u003c\/p\u003e\u003cp\u003eApplications Tested: This ALY7 antibody has been tested by flow cytometric analysis of LYVE-1\/GFP co-transfected cells. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells\/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.\u003c\/p\u003e\u003cp\u003eExcitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eFor Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.","brand":"Cytek Biosciences","offers":[{"title":"100 µg","offer_id":43190954360868,"sku":"12-0443-82","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/12-0443-lyve-1-flow-cytometry-07JAN2019LSRFSN-aly7-pe-abmaint_20190226153605.jpg?v=1769043354","url":"https:\/\/cytekbio.com\/ja\/products\/lyve1-monoclonal-antibody-aly7-pe-ebioscience","provider":"Cytek Biosciences","version":"1.0","type":"link"}