{"product_id":"il-5-monoclonal-antibody-trfk5-ebioscience","title":"IL-5 Monoclonal Antibody (TRFK5), eBioscience","description":"\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost: \u003c\/b\u003e\u003cspan\u003eRat\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype: \u003c\/b\u003e\u003cspan\u003eIgG1\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality: \u003c\/b\u003e\u003cspan\u003eMonoclonal\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone: \u003c\/b\u003e\u003cspan\u003eTRFK5\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity: \u003c\/b\u003e\u003cspan\u003eHu, Ms\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication: \u003c\/b\u003e\u003cspan\u003eFlow Cytometry\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution: \u003c\/b\u003e\u003cspan\u003eAssay-Dependent\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration: \u003c\/b\u003e\u003cspan\u003e0.5 mg\/mL\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage: \u003c\/b\u003e\u003cspan\u003e4°C\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation: \u003c\/b\u003e\u003cspan\u003ePBS with 0.09% sodium azide; pH 7.2\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification: \u003c\/b\u003e\u003cspan\u003eAffinity chromatography\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheet: \u003c\/b\u003e\u003ca href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/dataSheetPdf?producttype=antibody\u0026amp;productsubtype=antibody_primary\u0026amp;productId=14-7052-81\" target=\"_blank\"\u003eTDS\u003c\/a\u003e\u003c\/p\u003e \u003cbr\u003e\u003cp\u003e\u003cspan\u003e\u003cb\u003eSpecific Information\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003eDescription: The TRFK5 antibody reacts with mouse and human interleukin-5 (IL-5). The TRFK5 antibody is a neutralizing antibody. Mouse IL-5 is a disulfide-linked homodimer, containing two 113 amino acid peptides; these resolve as 32-34 kDa bands in SDS-PAGE. IL-5 is produced by T cells and has been known as eosinophil-differentiating factor (EDF), B cell growth factor II (BCGFII), and T cell-replacing factor (TRF). IL-5 induces eosinophil differentiation and promotes eosinophil survival and activation. In mice, IL-5 has been shown to stimulate B cell proliferation and antibody production.\u003c\/p\u003e\u003cp\u003eApplications Reported:  The TRFK5 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-5 bioactivity, IHC, and intracellular staining for flow cytometric analysis.\u003c\/p\u003e\u003cp\u003eApplications Tested: The TRFK5 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IL-5 in combination with the biotin TRFK4 (13-7051) antibody for detection and recombinant mouse IL-5 (14-8051) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg\/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg\/mL - 8 pg\/mL should be included in each ELISA plate. \u003c\/p\u003e\u003cp\u003e The TRFK5 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-5 in combination with the biotinylated JES1-5A10 (13-7059) antibody for detection and recombinant human IL-5 (14-80591) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg\/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg\/mL - 8 pg\/mL should be included in each ELISA plate.\u003c\/p\u003e\u003cp\u003eThe Functional Grade purified TRFK5 antibody has been tested for neutralization of IL-5 bioactivity.\u003c\/p\u003e\u003cp\u003ePurity: Greater than 90%, as determined by SDS-PAGE.\u003c\/p\u003e\u003cp\u003eAggregation:  Less than 10%, as determined by HPLC.\u003c\/p\u003e\u003cp\u003eFiltration: 0.2 µm post-manufacturing filtered.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eFor Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.","brand":"Cytek Biosciences","offers":[{"title":"50 µg","offer_id":43190936240164,"sku":"14-7052-81","price":131.0,"currency_code":"USD","in_stock":true},{"title":"100 µg","offer_id":43190936272932,"sku":"BMS179","price":321.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/Symbol_Full_Color_-_JPG_RGB_753224b2-12ed-45ff-8692-d32efcdb2b9e.jpg?v=1769043054","url":"https:\/\/cytekbio.com\/ja\/products\/il-5-monoclonal-antibody-trfk5-ebioscience","provider":"Cytek Biosciences","version":"1.0","type":"link"}