{"product_id":"hif-1-alpha-monoclonal-antibody-mgc3-apc-ebioscience","title":"HIF-1 alpha Monoclonal Antibody (Mgc3), APC, eBioscience","description":"\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost: \u003c\/b\u003e\u003cspan\u003eMouse\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype: \u003c\/b\u003e\u003cspan\u003eIgG1, kappa\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality: \u003c\/b\u003e\u003cspan\u003eMonoclonal\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone: \u003c\/b\u003e\u003cspan\u003eMgc3\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat: \u003c\/b\u003e\u003cspan\u003eAPC\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity: \u003c\/b\u003e\u003cspan\u003eHu, Ms\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication: \u003c\/b\u003e\u003cspan\u003eFlow Cytometry\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution: \u003c\/b\u003e\u003cspan\u003e1.0 µg\/test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration: \u003c\/b\u003e\u003cspan\u003e0.2 mg\/mL\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage: \u003c\/b\u003e\u003cspan\u003e4°C, store in dark, DO NOT FREEZE!\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation: \u003c\/b\u003e\u003cspan\u003ePBS with 0.09% sodium azide; pH 7.2\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification: \u003c\/b\u003e\u003cspan\u003eAffinity chromatography\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheet: \u003c\/b\u003e\u003ca href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/dataSheetPdf?producttype=antibody\u0026amp;productsubtype=antibody_primary\u0026amp;productId=17-7528-82\" target=\"_blank\"\u003eTDS\u003c\/a\u003e\u003c\/p\u003e \u003cbr\u003e\u003cp\u003e\u003cspan\u003e\u003cb\u003eSpecific Information\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003eDescription: This Mgc3 monoclonal antibody detects hypoxia-inducible factor 1 alpha (HIF-1 alpha) from human, non-human primate, bovine, mouse and porcine cells. This antibody does not cross-react with ARNT or the related HIF-2 alpha.\u003c\/p\u003e\u003cp\u003eMgc3 has been successfully used in western blotting, immunofluorescence, immunocytochemistry, immunoprecipitation, flow cytometry and gel shift procedures. By western blot, this antibody detects a ~93 kDa protein representing HIF-1 alpha after hypoxic induction in COS cells. Immunofluorescence staining of HIF-1 alpha in COS-7 cells with clone Mgc3 yields nuclear staining after exposing cells to 1% oxygen for 4 hours. In gel shift assay experiments, the Mgc3 antibody detected only mouse and not human HIF-1 alpha.\u003c\/p\u003e\u003cp\u003eOther anti-HIF1 alpha antibodies were previously included in a Thermo Scientific Cellomics High Content Screening Kit. Clone Mgc3 is now recommended as a replacement. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. High content assays may require further optimization, including the selection of the optimal Dylight-conjugated secondary antibody.\u003c\/p\u003e\u003cp\u003eApplications Reported: This Mgc3 antibody has been reported for use in flow cytometric analysis. \u003c\/p\u003e\u003cp\u003eApplications Tested: This Mgc3 antibody has been tested by flow cytometric analysis of DFOA stimulated HeLa cells using the methanol fixation protocol. This may be used at less than or equal to 1.0 µg\/mL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells\/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. \u003c\/p\u003e\u003cp\u003eExcitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eFor Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.","brand":"Cytek Biosciences","offers":[{"title":"100 µg","offer_id":43198613028900,"sku":"17-7528-82","price":443.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/17-7529-hif1alpha-flow-cytometry-13feb2019lsrf2rn-mgc3-apc-abmaint_20190307174206.jpg?v=1769203745","url":"https:\/\/cytekbio.com\/ja\/products\/hif-1-alpha-monoclonal-antibody-mgc3-apc-ebioscience","provider":"Cytek Biosciences","version":"1.0","type":"link"}