{"product_id":"cytek-cfluor-21-color-human-t-cell-kit","title":"Cytek\u003csup\u003e®\u003c\/sup\u003e cFluor\u003csup\u003e®\u003c\/sup\u003e 21-Color Human T-Cell Kit","description":"\u003cp\u003eThe Cytek\u003csup\u003e®\u003c\/sup\u003e cFluor\u003csup\u003e®\u003c\/sup\u003e 21-Color Human T-Cell Kit enables the identification and characterization of major human T cell (CD3+) subpopulations (CD4+, CD8+, and TCRγδ+) in human peripheral mononuclear cells (PBMCs) and whole blood. The reagents in this kit also support detailed analysis of CD3+CD4+ and CD3+CD8+ naive, central memory, effector memory, and effector subsets, as well as specialized CD3+CD4+ helper T cell subsets, including Th1, Th2, Th17, Th22, T follicular helper (Tfh), and T peripheral helper (Tph) cells. Activation and exhaustion markers, including CD38, HLA-DR, CD366 (TIM-3), and TIGIT, are incorporated to assess activation and functional states across all defined T cell subsets.\u003c\/p\u003e\n\u003cp\u003eThis panel has been optimized and titrated for robust and reproducible immunophenotyping of T cells in PBMCs and whole blood. It provides a multiparametric approach to characterize T cell subset distribution, functional status, and activation\/exhaustion phenotypes.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/Brochures\/Web_DMC-01004_Cytek-cFluor-21-Color-Human-T-Cell-Kit.pdf\" target=\"_blank\"\u003eDownload Flyer\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eTested Dilution:\u003c\/b\u003e\u003cspan\u003e  Use 5 µL \/ test for same-day, room-temperature protocols. For staining titers involving overnight incubation at 4°C, refer to the corresponding staining protocols.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eApplication:\u003c\/b\u003e\u003cspan\u003e Flow Cytometry\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eStorage: \u003c\/b\u003e\u003cspan\u003e2-\u003c\/span\u003e\u003cspan\u003e8°C and protected from Light. \u003c\/span\u003e\u003cem\u003eDo not freeze\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFormulation: \u003c\/strong\u003ePhosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)\u003cbr\u003eFor nanoparticle dye reagents: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/kit-documentation\/Cytek%C2%AE+cFluor%C2%AE+21-Color+Human+T-Cell+Kit\/R8-50012+Rev.+B_TDS_R7-40012+Cytek+cFluor%C2%AE+21-Color+Human+T-Cell+Kit.pdf\" rel=\"noopener\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/cFluor+SDS+Rev.+D+R9-70004.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eThe panel contains the following single vial reagents.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/cfluor\/images\/R7-40012_Table-1.png\" target=\"_blank\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/cfluor\/images\/R7-40012_Table-1.png\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1: \u003c\/strong\u003eComposition of the Cytek\u003csup\u003e®\u003c\/sup\u003e cFluor\u003csup\u003e®\u003c\/sup\u003e 21-Color Human T-Cell Kit\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDATA\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/cfluor\/images\/R7-40012-Figure-1.png\" target=\"_blank\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/cfluor\/images\/R7-40012-Figure-1.png\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/cfluor\/images\/R7-40012-Figure-1A.png\" target=\"_blank\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/cfluor\/images\/R7-40012-Figure-1A.png\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/cfluor\/images\/R7-40012-Figure-1B.png\" target=\"_blank\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/cfluor\/images\/R7-40012-Figure-1B.png\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1: \u003c\/strong\u003eT cell subpopulation gating hierarchy. Example of gating strategy using a whole blood sample. Lymphocytes were identified from CD45+ single cells. CD3+ and TCRγδ+ T cells were defined. The CD3+ T cells were then classified into CD4+ and CD8+ T cells. CD4+, CD8+, and TCRγδ+ T cells were subsequently partitioned into naive and memory T cells. \u003cstrong\u003eA)\u003c\/strong\u003e The memory CD4+ T cells were further divided into T helper subsets. \u003cstrong\u003eB)\u003c\/strong\u003e Memory CD8+ T cells were further divided into cytotoxic T subsets.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRECOMMENDED USAGE\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eHuman fresh whole blood (Heparin) and frozen PBMC samples have been tested to validate the performance of this kit. Please refer to the section below to download this kit's staining protocols, data acquisition protocols, experiment templates, and fluorochrome list.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eUse appropriate personal protective equipment per product safety data sheet when using this product.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDownload the Sample Preparation Guidelines for the Cytek\u003csup\u003e®\u003c\/sup\u003e cFluor\u003csup\u003e®\u003c\/sup\u003e 21-Color Human T-Cell Kit for\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/kit-documentation\/Cytek%C2%AE+cFluor%C2%AE+21-Color+Human+T-Cell+Kit\/DOC-00711+Rev.A_Sample+Preparation+(Whole+Blood)+Guidelines+for+Cytek+cFluor%C2%AE+21-Color+Human+T+cell+Kit+%E2%80%93+Same-Day+S.pdf\" target=\"_blank\"\u003eWhole Blood, Same-Day Room Temperature Staining\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/kit-documentation\/Cytek%C2%AE+cFluor%C2%AE+21-Color+Human+T-Cell+Kit\/DOC-00712+Rev.A_Sample+Preparation+(Whole+Blood)+Guidelines+for+Cytek+cFluor%C2%AE+21-Color+Human+T+cell+Kit+%E2%80%93+Overnight+.pdf\" target=\"_blank\"\u003eWhole Blood, Overnight 4°C Staining \u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/kit-documentation\/Cytek%C2%AE+cFluor%C2%AE+21-Color+Human+T-Cell+Kit\/DOC-00709+Rev.A_Sample+Preparation+(PBMC)+Guidelines+for+Cytek+cFluor%C2%AE+21-Color+Human+T+cell+Kit+%E2%80%93+Same-Day+Staining.pdf\" target=\"_blank\"\u003ePBMCs, Same-Day Room Temperature Staining\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/kit-documentation\/Cytek%C2%AE+cFluor%C2%AE+21-Color+Human+T-Cell+Kit\/DOC-00710+Rev.A_Sample+Preparation+(PBMC)+Guidelines+for+Cytek+cFluor%C2%AE+21-Color+Human+T+cell+Kit+%E2%80%93+Overnight+Stainin.pdf\" target=\"_blank\"\u003ePBMCs, Overnight 4°C Staining \u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eDownload the Acquisition Guidelines for the Cytek\u003csup\u003e®\u003c\/sup\u003e cFluor\u003csup\u003e®\u003c\/sup\u003e 21-Color Human T-Cell Kit for\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/kit-documentation\/Cytek%C2%AE+cFluor%C2%AE+21-Color+Human+T-Cell+Kit\/DOC-00708+Rev.A_Acquisition+(Whole+Blood)+Guidelines+for+Cytek+cFluor%C2%AE+21-Color+Human+T+cell+Kit+%E2%80%93+Same-Day+Staining.pdf\" target=\"_blank\"\u003eWhole Blood, Same-Day Room Temperature Staining\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/kit-documentation\/Cytek%C2%AE+cFluor%C2%AE+21-Color+Human+T-Cell+Kit\/DOC-00707+Rev.+A_Acquisition+(Whole+Blood)+Guidelines+for+Cytek+cFluor%C2%AE+21-Color+Human+T+cell+Kit+%E2%80%93+Overnight+Staini.pdf\" target=\"_blank\"\u003eWhole Blood, Overnight 4°C Staining \u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/kit-documentation\/Cytek%C2%AE+cFluor%C2%AE+21-Color+Human+T-Cell+Kit\/DOC-00706+Rev.A_Acquisition+(PBMC)+Guidelines+for+Cytek+cFluor%C2%AE+21-Color+Human+T+cell+Kit+%E2%80%93+Same-Day+%26+Overnight+Staining+Protocol.pdf\" target=\"_blank\"\u003ePBMCs, Same-Day Room Temperature and Overnight 4°C Staining\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eDownload the Experiment Template for the Cytek\u003csup\u003e®\u003c\/sup\u003e cFluor\u003csup\u003e®\u003c\/sup\u003e 21-Color Human T-Cell Kit for\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/kit-documentation\/Cytek%C2%AE+cFluor%C2%AE+21-Color+Human+T-Cell+Kit\/Cytek+T+cell+Kit+Template_Blood_Same-Day.zip\" target=\"_blank\"\u003eWhole Blood, Same-Day Room Temperature Staining\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/kit-documentation\/Cytek%C2%AE+cFluor%C2%AE+21-Color+Human+T-Cell+Kit\/Cytek+T+cell+Kit+Template_Blood_Overnight.zip\" target=\"_blank\"\u003eWhole Blood, Overnight 4°C Staining \u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/kit-documentation\/Cytek%C2%AE+cFluor%C2%AE+21-Color+Human+T-Cell+Kit\/Cytek+T+cell+Kit+Template_PBMC.zip\" target=\"_blank\"\u003ePBMCs, Same-Day Room Temperature and Overnight 4°C Staining\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eDownload the \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/kit-documentation\/Cytek%C2%AE+cFluor%C2%AE+21-Color+Human+T-Cell+Kit\/Cytek%C2%AE+T+cell+Kit+Tags.CSV\" target=\"_blank\"\u003eCytek\u003csup\u003e®\u003c\/sup\u003e cFluor\u003csup\u003e®\u003c\/sup\u003e 21-Color Human T-Cell Kit Assay Tags\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eREFERENCES\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eMousset C.M., et al. 2019. Cytometry A. 95:647\u003c\/p\u003e\n\u003cp\u003eYoshitomi H., et al. 2020. Cell. Mol. Immunol. 18:523\u003c\/p\u003e\n\u003cp\u003eRasouli J., et al. 2015. J of Immunol. 194:5085\u003c\/p\u003e\n\u003cp\u003eLaidlaw B.J., et al. 2016. Nat. Rev. Immunol. 16:102\u003c\/p\u003e\n\u003cp\u003eGolubovskaya V., et al. 2016. Cancers. 8:36\u003c\/p\u003e\n\u003cp\u003eHu Y., et al. 2023. Signal Transduct. Target.Ther. 8:434\u003c\/p\u003e\n\u003cp\u003eBaessler A., et al. 2024. Annu. Rev. Immunol. 42:179\u003c\/p\u003e\n\u003cp\u003eMosopust D., et al. 2026. Nat. Rev. Immunol. 26:298 \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFor Research Use Only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003ecFluor\u003csup\u003e®\u003c\/sup\u003e V547, cFluor\u003csup\u003e®\u003c\/sup\u003e B515, cFluor\u003csup\u003e®\u003c\/sup\u003e B548, cFluor\u003csup\u003e®\u003c\/sup\u003e BYG610, cFluor\u003csup\u003e®\u003c\/sup\u003e R685, cFluor\u003csup\u003e®\u003c\/sup\u003e R720, and cFluor\u003csup\u003e®\u003c\/sup\u003e R840 are equivalent to CF\u003csup\u003e®\u003c\/sup\u003e405L, CF\u003csup\u003e®\u003c\/sup\u003e488A, CF\u003csup\u003e®\u003c\/sup\u003e514, RPE-CF\u003csup\u003e®\u003c\/sup\u003e596R, CF\u003csup\u003e®\u003c\/sup\u003e660C, CF\u003csup\u003e®\u003c\/sup\u003e700, and APC-CF\u003csup\u003e®\u003c\/sup\u003e790T respectively, manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.\u003c\/p\u003e\n\u003cp\u003ecFluor\u003csup\u003e®\u003c\/sup\u003e UV395, cFluor\u003csup\u003e®\u003c\/sup\u003e V435, cFluor\u003csup\u003e®\u003c\/sup\u003e V605, cFluor\u003csup\u003e®\u003c\/sup\u003e V670, cFluor\u003csup\u003e®\u003c\/sup\u003e V715, and cFluor\u003csup\u003e®\u003c\/sup\u003e V780 are StarBright™ UltraViolet 400 Dye, StarBright™ Violet 440 Dye, StarBright™ Violet 610 Dye, StarBright™ Violet 670 Dye, StarBright™ Violet 710 Dye, and StarBright™ Violet 790 Dye respectively, manufactured and provided by Bio-Rad, Inc.\u003c\/p\u003e\n\u003cp\u003eStarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.\u003c\/p\u003e\n\u003cp\u003ecFluor\u003csup\u003e®\u003c\/sup\u003e BYG610, cFluor\u003csup\u003e®\u003c\/sup\u003e BYG667, cFluor\u003csup\u003e®\u003c\/sup\u003e BYG710, cFluor\u003csup\u003e®\u003c\/sup\u003e BYG750, and cFluor\u003csup\u003e®\u003c\/sup\u003e BYG781 are tandem dyes made with R-PE. cFluor\u003csup\u003e®\u003c\/sup\u003e R780 and cFluor\u003csup\u003e®\u003c\/sup\u003e R840 are tandem dyes made with APC. cFluor\u003csup\u003e®\u003c\/sup\u003e V605, cFluor\u003csup\u003e®\u003c\/sup\u003e V670, cFluor\u003csup\u003e®\u003c\/sup\u003e V715, and cFluor\u003csup\u003e®\u003c\/sup\u003e V780 are tandem dyes. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.\u003c\/p\u003e","brand":"Cytek Biosciences","offers":[{"title":"Default Title","offer_id":43933686726692,"sku":"R7-40012","price":3933.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/R7-40012_web.png?v=1774983436","url":"https:\/\/cytekbio.com\/ja\/products\/cytek-cfluor-21-color-human-t-cell-kit","provider":"Cytek Biosciences","version":"1.0","type":"link"}