{"product_id":"alexa-fluor-700-anti-human-cd274-pd-l1-b7-h1-29e-2a3","title":"Alexa Fluor\u003csup\u003e\u0026trade;\u003c\/sup\u003e 700 Anti-Human CD274 (PD-L1, B7-H1) (29E.2A3)","description":"\u003cp\u003eThe mouse monoclonal antibody clone 29E.2A3 binds to human CD274, also known as PD-L1 or B7-H1. CD274 is a type 1 transmembrane glycoprotein and a member of the Ig superfamily. PD-L1 is a ligand for CD279 (PD-1) and plays an important role in regulating immune cell responses. This binding plays a role in clonal selection, elimination of autoreactive lymphocytes, and tolerance development. Additionally, PD-L1 can limit the activation of mature T cells. As part of the B7 ligand family, its interactions with the CD28 receptor family, including CTLA-4, are crucial for balancing T cell activation and tolerance. CD274 is expressed on activated monocytes, macrophages, dendritic cells, and T cells.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: 29E.2A3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: CD274 (PD-L1, B7-H1)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: Alexa Fluor™ 700\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Mouse IgG2b, k\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution\u003c\/b\u003e: 5 µL\/Test\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: FC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light.\u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/project_elva_2k\/R8-94565+Rev.+A_TDS_Alexa+Fluor+700+Anti-Human+CD274+(PD-L1+B7-H1)+(29E.2A3).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eFlow cytometry:  The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eREFERENCES\u003c\/b\u003e\u003c\/p\u003e\u003col\u003e\u003cli\u003eLatchman et al. 2001. Nat Immunol. 2:261-8.\u003c\/li\u003e\u003c\/ol\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e“Alexa Fluor“ is a trademark of Thermo Fisher Scientific Inc.","brand":"Cytek Biosciences","offers":[{"title":"100 Tests","offer_id":67679268241444,"sku":"R7-94565","price":292.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/R7-94565_FC_Profile.png?v=1792019654","url":"https:\/\/cytekbio.com\/ja\/products\/alexa-fluor-700-anti-human-cd274-pd-l1-b7-h1-29e-2a3","provider":"Cytek Biosciences","version":"1.0","type":"link"}