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Fueling Scientific Discovery through Technological Breakthroughs

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Optics

EXCITATION OPTICS

OPTICAL PLATFORM

Aurora contains a fixed optical assembly with the capacity to be configured with up to five spatially separated laser beams. Laser delays are automatically adjusted during instrument QC.

LASERS

Base model three-laser configuration: 405 nm: 100 mW, 488 nm: 50 mW, 640 nm: 80 mW

Available laser upgrades: 355 nm: 20 mW, 561 nm: 50 mW

BEAM GEOMETRY

Flat-Top laser beam profile with narrow vertical beam height optimized for small particle detection.

EMISSION OPTICS

EMISSION COLLECTION

Fused silica cuvette coupled to high NA lens for optimum collection efficiency to optical fibers.

FORWARD AND SIDE SCATTER DETECTION

FSC: high-performance semiconductor detector with 488nm bandpass filter

SSC: two high-performance semiconductor detectors with 405nm and 488nm bandpass filters

FLUORESCENCE DETECTORS

Proprietary high sensitivity Coarse Wavelength Division Multiplexing (CWDM) semiconductor array per laser enabling more efficient spectrum capture in the 365-830 nm range. No filter changes required for any fluorochrome excited by the 355 nm, 405 nm, 488 nm, 561 nm, 640 nm lasers.

STANDARD OPTICAL CONFIGURATION

Violet detector module: 16 channels unevenly spaced bandwidth from 420-830 nm.

Blue detector module:14 channels unevenly spaced bandwidth from 500-830 nm.

Red detector module: 8 channels unevenly spaced bandwidth from 650-830 nm.

4 and 5 Laser Options:

Yellow-Green detector module: 10 channels unevenly spaced bandwidth from 570-830 nm.

Ultraviolet detector module: 16 channels unevenly spaced bandwidth from 365-830 nm.

Fluidics

SAMPLE FLOW RATES

Low: 15 μL/min, Medium: 30 μL/min, High: 60 μL/min, Plate high-throughput mode: 100 μL/min

FLUIDIC MODES

Long clean, SIT flush, Purge filter, Clean flow cell

MANUAL SAMPLE INPUT FORMATS

12x75 mm polystyrene and polypropylene tubes

STANDARD FLUIDIC RESERVOIRS

4L fluid container set with level-sensing provided. Compatible with 20 L sheath and waste cubitainers.

VOLUMETRIC SENSOR

Volumetric measurement during sample recording enables calculation of counts per μL for any gated population.

PLATE LOADER OPTION

96-well microtiter plate capabilityThroughput time 35 minutes at High Throughput mode sampling 7 μL/wellPlate stage temperature: 4-30°C

PLATE LOADER CARRYOVER

Default mode: ≤0.3%, Low Carryover mode: ≤0.1%, High Throughput mode: ≤1%

Performance

FLUORESCENCE SENSITIVITY

FITC: <110 MEFL, PE: <35 MEFL, APC: <15 MEFL, Pacific Blue: <200MEFL*Measurements based on an average from three systems and performed using SPHERO Rainbow Calibration Particle (RCP-30-5A) based on its peak emission channel.

FLUORESCENCE LINEARITY

FITC R2 ≥0.995 / PE R2 ≥0.995

FORWARD AND SIDE SCATTER

RESOLUTION

Performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

SIDE SCATTER RESOLUTION

Capable of resolving 0.2 μm beads from noise.

CARRYOVER

≤0.1%

DATA ACQUISITION RATE

35,000 events/s*

*Three laser system

Software

SPECTROFLO

®

SOFTWARE

Live unmixing during acquisitionDeveloped specifically to streamline assay setup, data acquisition, and file exportAutomated QC module Autofluorescence extractionRaw and Unmixed FCS 3.1 files

Electronics

SIGNAL PROCESSING

Digital signal processing with automatic window gate adjustment.22-bit 6.5 log decades. Threshold using any single parameter or combination of parameters.

PULSE SHAPE PARAMETERS

Pulse Area and Height for every parameter. Width for scatter parameters and one fluorescence parameter for each laser.

Workstation

Workstation specifications may vary between laser configuration; below is for three-laser configuration.

OPERATING SYSTEM

Windows® 10 Pro 64-bit

PROCESSOR

Intel® Core™ i7 processor, 3.2 GHz

RAM

16 GB

HARD DRIVE

500GB SSD / 1 TB SATA

VIDEO PROCESSOR

NVIDIA® GeForce

MONITOR

32” UHD 4K Monitor

Installation Requirements

Dimensions (W x D X H)

INSTRUMENT DIMENSIONS

Without loader: 54 x 52 x 52 cmWith loader: 58.4 x 62 x 52 cm

INSTRUMENT WEIGHT

Instrument weight (4 lasers): 80 kgLoader weight: 13 kg

COMPUTER DIMENSIONS

29.1 x 9.25 x 34.4 cm

RECOMMENDED WORKSPACE

157 x 71 x 132 cm

Room Requirements

POWER

100-240 V, 50/60 Hz, 2A max

HEAT DISSIPATION

500 W with all solid-state lasers

TEMPERATURE

15–28°C

HUMIDITY

20%-85% relative non-condensing

AIR FILTERING

No excessive dust or smoke

LIGHTING

No special requirements

Regulatory Status

For Research Use Only. Not for use in diagnostic or therapeutic procedures.