cFluor® UV515 anti-Human CD45RO (UCHL1)
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Description
The UCHL1 antibody reacts with the human CD45 isoform known as CD45RO, a 220-kDa protein tyrosine phosphatase. CD45 is one of the most abundant hematopoietic markers and is expressed on all leukocytes (leukocyte common antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, and others. The UCHL1 antibody is widely used as a marker for human CD45RO expression on thymocytes, activated memory T cells, monocytes, macrophages, and granulocytes. The antibody is reported to be cross-reactive for chimpanzee CD45RO.
The antibody was purified by affinity chromatography and conjugated to the fluorophore.
PRODUCT DETAILS
Clone: UCHL1
Reactivity: Human
Format: cFluor® UV515
Isotype: Mouse IgG2a
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from light. Do not freeze.
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)
EXAMPLE DATA
cFluor® UV515 anti-Human CD45RO (UCHL1), (right) vs. Isotype Control (left). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum.
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
For Research Use Only. Not intended for use in diagnostic procedures.
*Cytek® cFluor® UV515 is StarBright™ UltraViolet 510 Dye, manufactured and provided by Bio-Rad, Inc.
StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.