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cFluor<sup>&reg;</sup> UV515 anti-Human CD33 (WM53)

cFluor® UV515 anti-Human CD33 (WM53)

SKU R7-20851
Regular price

$478.00

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Description

The WM53 monoclonal antibody binds to human CD33, a 67-kDa type I transmembrane glycoprotein in the immunoglobulin family. CD33, also known as Siglec-3, glycoprotein 67 (gp67), and p67, is expressed on monocytes, activated T cells, myeloid progenitors, granulocytes, dendritic cells, and mast cells. It is absent on erythrocytes, platelets, and lymphoid cells. CD33 binds to sialic acids to act as a sialic acid-dependent cell adhesion molecule. It also contains intracellular tyrosine-based inhibition motifs (ITIMs), suggesting it functions to inhibit cellular activity.
The antibody was purified by affinity chromatography and conjugated to the fluorophore.


PRODUCT DETAILS

Clone: WM53

Reactivity: Human

Format: cFluor® UV515

Isotype: Mouse IgG1

Tested Dilution: 5 μL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze.

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS SDS



EXAMPLE DATA


Example data for cFluor® UV515 anti-Human CD33 (WM53)

cFluor® UV515 anti-Human CD33 (WM53), (right) vs. Isotype Control (left). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum.



RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.


Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.


For Research Use Only. Not intended for use in diagnostic procedures.


*Cytek® cFluor® UV515 is StarBright™ UltraViolet 510 Dye, manufactured and provided by Bio-Rad, Inc.

StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.

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