cFluor® UV440 anti-Human CD69 (FN50)
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Description
The FN50 monoclonal antibody reacts with human CD69, a type II transmembrane glycoprotein also known as the very early activation antigen (VEA), EA-1, Leu23, activation inducer molecule (AIM), and C-type lectin domain family 2, member C (CLEC2C). CD69 is expressed as a disulfide-linked homodimer on activated T and B cells, NK cells, neutrophils, and monocytes. Induction occurs rapidly upon activation and is transient. It is also constitutively expressed on platelets and a subset of thymocytes (Gavioli R, et al. 1992. Cell Immunol. 142(1):186-96).
The antibody was purified by affinity chromatography and conjugated to the fluorophore.
PRODUCT DETAILS
Clone: FN50
Reactivity: Human
Format: cFluor® UV440
Isotype: Mouse IgG1
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from light. Do not freeze.
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)
EXAMPLE DATA
cFluor® UV440 anti-Human CD69 (FN50), (right) vs. Isotype Control (left). All experiments performed on PBMCs extracted from human blood stimulated with PHA and LPS for 48 hours, gated on live single cell lymphocytes, in the presence of 10% human serum.
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
For Research Use Only. Not intended for use in diagnostic procedures.
*Cytek® cFluor® UV440 is StarBright™ UltraViolet 445 Dye, manufactured and provided by Bio-Rad, Inc.
StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.